Josh Powell is in a critical condition in hospital with his 18-month-old child after his family’s people carrier and a heavy goods vehicle collided.
Wife Zoe Powell, 29, and daughters Amelia, four, and Phoebe, eight, and six-year-old son Simeon died in the incident on Monday evening.
The HGV driver, a 56-year-old man, suffered minor injuries.
The Just Giving crowdfunder, set up by Andrew Welsford, states: “This (is) such a sad and tragic loss for a work colleague on the railway, whose family have been taken from him in a tragic car crash near Oxford.
“Many people in the world of the Orange Army (rail workers, who use high-vis jackets) will know Josh and I hope will contribute to give him some financial support when he needs (it) later in recovery.”
It raised £59,713 by Thursday morning, well past the initial goal of £1,000, with more than 1,600 people donating.
Watch: Community reacts to tragic Oxford car crash
He said Josh Powell is a contractor in the railway industry.
Tributes left to the family were made by people in Chinnor, where they lived.
A neighbour who did not want to be named said: “They stayed here after the overnight fire – there was smoke pouring from their house.
“It’s incredibly sad, it was a hard enough year for them regardless, and now this… it’s just incredible.
“It’s rather raw and it’s horrendous. They were all absolutely lovely – the children were a delight, they were bouncy, bubbly, just really happy children.”
Reverend Dr Jacky Barr, of St Andrew’s Church in Chinnor, where the family attended, said: “They were just a delightful family. The children were always very engaging.
“They were just a lovely family, they really were. This has come as such a shock and loss to us all in the area.”
It also emerged on Wednesday that the family had recently endured a house fire and were moving around before finding a long-term place.
Sergeant Dominic Mahon, of Thames Valley Police, said the crash scene was “incredibly upsetting” and urged anyone who saw what happened to contact police.
Watch: What is the Bounce Back scheme?